diff --git a/pkgs/applications/graphics/hydrus/default.nix b/pkgs/applications/graphics/hydrus/default.nix
index 954f97bf9b4a..bc9d1a82a0f3 100644
--- a/pkgs/applications/graphics/hydrus/default.nix
+++ b/pkgs/applications/graphics/hydrus/default.nix
@@ -10,14 +10,14 @@
 
 python3Packages.buildPythonPackage rec {
   pname = "hydrus";
-  version = "493";
+  version = "495";
   format = "other";
 
   src = fetchFromGitHub {
     owner = "hydrusnetwork";
     repo = "hydrus";
     rev = "refs/tags/v${version}";
-    sha256 = "sha256-sROmWFH3sDBDh1VSVLTM71Y9qD8CndvwW7PKzkavIuc=";
+    sha256 = "sha256-MuSK19oSrRJZebATRpDW1CmUq4NKcpuoPmOuLyvL8A8=";
   };
 
   nativeBuildInputs = [
diff --git a/pkgs/applications/networking/p2p/zeronet-conservancy/default.nix b/pkgs/applications/networking/p2p/zeronet-conservancy/default.nix
index 951e42475e18..f04a3e07a052 100644
--- a/pkgs/applications/networking/p2p/zeronet-conservancy/default.nix
+++ b/pkgs/applications/networking/p2p/zeronet-conservancy/default.nix
@@ -6,19 +6,20 @@
 
 python3Packages.buildPythonApplication rec {
   pname = "zeronet-conservancy";
-  version = "0.7.6";
+  version = "0.7.7";
   format = "other";
 
   src = fetchFromGitHub {
     owner = "zeronet-conservancy";
     repo = "zeronet-conservancy";
     rev = "v${version}";
-    sha256 = "sha256-tWNU7UJVWB+aRLam6WKV/HaRRTIQvlEgxe4xJYKpXJY=";
+    sha256 = "sha256-6qBdq6DoIKZUUGflz7kWu3S3pMJB4vkGUytpU5EatP0=";
   };
 
   propagatedBuildInputs = with python3Packages; [
     gevent msgpack base58 merkletools rsa pysocks pyasn1 websocket-client
-    gevent-websocket rencode bitcoinlib maxminddb pyopenssl rich
+    gevent-websocket rencode bitcoinlib maxminddb pyopenssl rich defusedxml
+    pyaes
   ];
 
   buildPhase = ''
diff --git a/pkgs/applications/science/biology/samtools/default.nix b/pkgs/applications/science/biology/samtools/default.nix
index ec61e0bd7ce4..29ba667b0552 100644
--- a/pkgs/applications/science/biology/samtools/default.nix
+++ b/pkgs/applications/science/biology/samtools/default.nix
@@ -18,6 +18,9 @@ stdenv.mkDerivation rec {
     })
   ];
 
+  # tests require `bgzip` from the htslib package
+  checkInputs = [ htslib ];
+
   nativeBuildInputs = [ perl ];
 
   buildInputs = [ zlib ncurses htslib ];
diff --git a/pkgs/applications/video/freetube/default.nix b/pkgs/applications/video/freetube/default.nix
index 3ceda283d3d8..b507c0d31947 100644
--- a/pkgs/applications/video/freetube/default.nix
+++ b/pkgs/applications/video/freetube/default.nix
@@ -2,11 +2,11 @@
 
 stdenv.mkDerivation rec {
   pname = "freetube";
-  version = "0.17.0";
+  version = "0.17.1";
 
   src = fetchurl {
     url = "https://github.com/FreeTubeApp/FreeTube/releases/download/v${version}-beta/freetube_${version}_amd64.AppImage";
-    sha256 = "sha256-OlWNln62VouUJzzk0CtED+OdSM+aBc4NOu1TSaKVWnk=";
+    sha256 = "1n5r1h2khjwdsckiviv8f2pflxibk8rs68fs08jak0kbm0kkyj18";
   };
 
   appimageContents = appimageTools.extractType2 {
diff --git a/pkgs/applications/video/mpv/scripts/mpvacious.nix b/pkgs/applications/video/mpv/scripts/mpvacious.nix
index 9ddd6e308c3b..071f762ff4a7 100644
--- a/pkgs/applications/video/mpv/scripts/mpvacious.nix
+++ b/pkgs/applications/video/mpv/scripts/mpvacious.nix
@@ -18,10 +18,7 @@ stdenvNoCC.mkDerivation rec {
   };
 
   postPatch = ''
-    # 'require' replaced with 'dofile' to work around
-    # https://github.com/mpv-player/mpv/issues/7399 until fixed in mpvacious
     substituteInPlace subs2srs.lua \
-      --replace "require('osd_styler')" "dofile('"$out/share/mpv/scripts/mpvacious/osd_styler.lua"')" \
       --replace "'curl'" "'${curl}/bin/curl'" \
       --replace "'wl-copy'" "'${wl-clipboard}/bin/wl-copy'" \
       --replace "xclip" "${xclip}/bin/xclip"
@@ -36,7 +33,7 @@ stdenvNoCC.mkDerivation rec {
     runHook postInstall
   '';
 
-  passthru.scriptName = "mpvacious/subs2srs.lua";
+  passthru.scriptName = "mpvacious";
 
   meta = with lib; {
     description = "Adds mpv keybindings to create Anki cards from movies and TV shows";
diff --git a/pkgs/games/steam/runtime.nix b/pkgs/games/steam/runtime.nix
index 8f2398255a41..c8c67e94b4a4 100644
--- a/pkgs/games/steam/runtime.nix
+++ b/pkgs/games/steam/runtime.nix
@@ -8,11 +8,11 @@ stdenv.mkDerivation rec {
 
   pname = "steam-runtime";
   # from https://repo.steampowered.com/steamrt-images-scout/snapshots/latest-steam-client-general-availability/VERSION.txt
-  version = "0.20211102.0";
+  version = "0.20220601.1";
 
   src = fetchurl {
     url = "https://repo.steampowered.com/steamrt-images-scout/snapshots/${version}/steam-runtime.tar.xz";
-    sha256 = "sha256-/ve4oVxKQ4uTaTiTg8Qc0Kyb4GRJKGZ5SQVLIyeJSpI=";
+    sha256 = "sha256-uYauNtbUlvrnATGks7hWy1zt4Y7AEeADrCr1eVylPbY=";
     name = "scout-runtime-${version}.tar.gz";
   };