diff --git a/pkgs/by-name/fu/fuseiso/package.nix b/pkgs/by-name/fu/fuseiso/package.nix
deleted file mode 100644
index 5effa1a7520e..000000000000
--- a/pkgs/by-name/fu/fuseiso/package.nix
+++ /dev/null
@@ -1,76 +0,0 @@
-{
-  lib,
-  stdenv,
-  fetchurl,
-  fetchpatch,
-  autoreconfHook,
-  pkg-config,
-  fuse,
-  glib,
-  zlib,
-}:
-
-stdenv.mkDerivation rec {
-  pname = "fuseiso";
-  version = "20070708";
-
-  src = fetchurl {
-    url = "mirror://sourceforge/project/fuseiso/fuseiso/${version}/fuseiso-${version}.tar.bz2";
-    sha256 = "127xql52dcdhmh7s5m9xc6q39jdlj3zhbjar1j821kb6gl3jw94b";
-  };
-
-  patches = [
-    (fetchpatch {
-      name = "00-support_large_iso.patch";
-      url = "https://sources.debian.net/data/main/f/fuseiso/${version}-3.2/debian/patches/00-support_large_iso.patch";
-      sha256 = "1lmclb1qwzz5f4wlq693g83bblwnjjl73qhgfxbsaac5hnn2shjw";
-    })
-    (fetchpatch {
-      name = "01-fix_typo.patch";
-      url = "https://sources.debian.net/data/main/f/fuseiso/${version}-3.2/debian/patches/01-fix_typo.patch";
-      sha256 = "14rpxp0yylzsgqv0r19l4wx1h5hvqp617gpv1yg0w48amr9drasa";
-    })
-    (fetchpatch {
-      name = "02-prevent-buffer-overflow_CVE-2015-8837.patch";
-      url = "https://sources.debian.net/data/main/f/fuseiso/${version}-3.2/debian/patches/02-prevent-buffer-overflow.patch";
-      sha256 = "1ls2pp3mh91pdb51qz1fsd8pwhbky6988bpd156bn7wgfxqzh8ig";
-    })
-    (fetchpatch {
-      name = "03-prevent-integer-overflow_CVE-2015-8836.patch";
-      url = "https://sources.debian.net/data/main/f/fuseiso/${version}-3.2/debian/patches/03-prevent-integer-overflow.patch";
-      sha256 = "100cw07fk4sa3hl7a1gk2hgz4qsxdw99y20r7wpidwwwzy463zcv";
-    })
-  ];
-
-  nativeBuildInputs = [
-    autoreconfHook
-    pkg-config
-  ];
-
-  buildInputs = [
-    fuse
-    glib
-    zlib
-  ];
-
-  # after autoreconfHook, glib and zlib are not found, so force link against
-  # them
-  env.NIX_LDFLAGS = toString [
-    "-lglib-2.0"
-    "-lz"
-  ];
-
-  enableParallelBuilding = true;
-
-  postInstall = ''
-    install -Dm444 -t $out/share/doc/${pname} NEWS README
-  '';
-
-  meta = {
-    description = "FUSE module to mount ISO filesystem images";
-    homepage = "https://sourceforge.net/projects/fuseiso";
-    license = lib.licenses.gpl2Plus;
-    platforms = lib.platforms.linux;
-    mainProgram = "fuseiso";
-  };
-}
diff --git a/pkgs/by-name/q4/q4wine/package.nix b/pkgs/by-name/q4/q4wine/package.nix
index bae5afc03705..223429dc4022 100644
--- a/pkgs/by-name/q4/q4wine/package.nix
+++ b/pkgs/by-name/q4/q4wine/package.nix
@@ -7,7 +7,6 @@
   qt6,
   icoutils, # build and runtime deps.
   wget,
-  fuseiso,
   wine,
   which, # runtime deps.
 }:
@@ -43,7 +42,6 @@ stdenv.mkDerivation (finalAttrs: {
         lib.makeBinPath [
           icoutils
           wget
-          fuseiso
           wine
           which
         ]
diff --git a/pkgs/top-level/aliases.nix b/pkgs/top-level/aliases.nix
index 7d9827cb7651..860584e73a64 100644
--- a/pkgs/top-level/aliases.nix
+++ b/pkgs/top-level/aliases.nix
@@ -821,6 +821,7 @@ mapAliases {
   frugal = throw "'frugal' was removed because upstream has been pulled"; # Added 2025-12-20
   fuse-7z-ng = throw "'fuse-7z-ng' was removed as it is unmaintained, and depends on fuse2"; # Added 2026-05-05
   fusee-launcher = throw "'fusee-launcher' was removed as upstream removed the original source repository fearing legal repercussions"; # Added 2025-07-05
+  fuseiso = throw "'fuseiso' has been removed as it is unmaintained upstream, and depends on fuse2"; # Added 2026-05-05
   fusionInventory = warnAlias "'fusionInventory' has been renamed to 'fusioninventory-agent'" fusioninventory-agent; # Added 2026-02-08
   futuresql = throw "'futuresql' has been renamed to/replaced by 'libsForQt5.futuresql'"; # Converted to throw 2025-10-27
   fx_cast_bridge = throw "'fx_cast_bridge' has been renamed to/replaced by 'fx-cast-bridge'"; # Converted to throw 2025-10-27