diff --git a/pkgs/development/python-modules/cyvcf2/default.nix b/pkgs/development/python-modules/cyvcf2/default.nix
new file mode 100644
index 000000000000..a29f7f21b2b7
--- /dev/null
+++ b/pkgs/development/python-modules/cyvcf2/default.nix
@@ -0,0 +1,89 @@
+{
+  lib,
+  buildPythonPackage,
+  fetchFromGitHub,
+  python,
+
+  # build-system
+  cmake,
+  cython,
+  ninja,
+  numpy,
+  scikit-build-core,
+
+  # nativeBuildInputs
+  pkg-config,
+
+  # buildInputs
+  htslib,
+
+  # dependencies
+  click,
+  coloredlogs,
+
+  # tests
+  pytestCheckHook,
+}:
+
+buildPythonPackage (finalAttrs: {
+  pname = "cyvcf2";
+  version = "0.34.0";
+  pyproject = true;
+  __structuredAttrs = true;
+
+  src = fetchFromGitHub {
+    owner = "brentp";
+    repo = "cyvcf2";
+    tag = "v${finalAttrs.version}";
+    hash = "sha256-XDo2cEcZm5y6u9Gc2e7q2tg8pt3C/5cmnWRe6Dr5x7M=";
+  };
+
+  build-system = [
+    cmake
+    cython
+    ninja
+    numpy
+    scikit-build-core
+  ];
+  dontUseCmakeConfigure = true;
+
+  nativeBuildInputs = [
+    pkg-config
+  ];
+
+  buildInputs = [
+    htslib
+  ];
+
+  env = {
+    # Link against the system htslib instead of building the bundled submodule
+    CYVCF2_HTSLIB_MODE = "EXTERNAL";
+  };
+
+  dependencies = [
+    click
+    coloredlogs
+    numpy
+  ];
+
+  pythonImportsCheck = [ "cyvcf2" ];
+
+  nativeCheckInputs = [
+    pytestCheckHook
+  ];
+
+  # The test suite lives inside the package directory and relies on relative imports
+  # (`from ..cyvcf2 import VCF`) as well as data files next to it, so it must run from the source
+  # tree. Bring the compiled extension along.
+  preCheck = ''
+    cp $out/${python.sitePackages}/cyvcf2/cyvcf2*.so cyvcf2/
+  '';
+
+  meta = {
+    description = "Cython wrapper around htslib built for fast parsing of Variant Call Format (VCF) files";
+    homepage = "https://github.com/brentp/cyvcf2";
+    changelog = "https://github.com/brentp/cyvcf2/blob/${finalAttrs.src.rev}/CHANGES.md";
+    license = lib.licenses.mit;
+    maintainers = with lib.maintainers; [ GaetanLepage ];
+  };
+})
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index d8ba8c31a6e0..38c9e74c71e9 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -3983,6 +3983,8 @@ self: super: with self; {
 
   cytoolz = callPackage ../development/python-modules/cytoolz { };
 
+  cyvcf2 = callPackage ../development/python-modules/cyvcf2 { };
+
   cyvest = callPackage ../development/python-modules/cyvest { };
 
   dacite = callPackage ../development/python-modules/dacite { };