diff --git a/pkgs/applications/audio/quodlibet/default.nix b/pkgs/applications/audio/quodlibet/default.nix
index 7d0703e47c08..6169917a7d09 100644
--- a/pkgs/applications/audio/quodlibet/default.nix
+++ b/pkgs/applications/audio/quodlibet/default.nix
@@ -16,7 +16,7 @@
   glib,
   glib-networking,
   gtk3,
-  gtksourceview,
+  gtksourceview3,
   kakasi,
   keybinder3,
   libappindicator,
@@ -89,7 +89,7 @@ python3.pkgs.buildPythonApplication (finalAttrs: {
     glib
     glib-networking
     gtk3
-    gtksourceview
+    gtksourceview3
     kakasi
     keybinder3
     libappindicator
diff --git a/pkgs/by-name/de/denemo/package.nix b/pkgs/by-name/de/denemo/package.nix
index 80f7970597a5..92734fa6af91 100644
--- a/pkgs/by-name/de/denemo/package.nix
+++ b/pkgs/by-name/de/denemo/package.nix
@@ -15,7 +15,7 @@
   libsndfile,
   aubio,
   gtk3,
-  gtksourceview,
+  gtksourceview3,
   evince,
   fluidsynth,
   rubberband,
@@ -47,7 +47,7 @@ stdenv.mkDerivation (finalAttrs: {
     libsndfile
     aubio
     gtk3
-    gtksourceview
+    gtksourceview3
     evince
     fluidsynth
     rubberband
diff --git a/pkgs/by-name/lu/lutris/package.nix b/pkgs/by-name/lu/lutris/package.nix
index d40cd205f224..2ec975da987d 100644
--- a/pkgs/by-name/lu/lutris/package.nix
+++ b/pkgs/by-name/lu/lutris/package.nix
@@ -18,7 +18,7 @@ let
   gnomeDeps =
     pkgs: with pkgs; [
       zenity
-      gtksourceview
+      gtksourceview3
       gnome-desktop
       libgnome-keyring
       webkitgtk_4_1
diff --git a/pkgs/by-name/ma/marker/package.nix b/pkgs/by-name/ma/marker/package.nix
index f22d93f73584..b0824a79952c 100644
--- a/pkgs/by-name/ma/marker/package.nix
+++ b/pkgs/by-name/ma/marker/package.nix
@@ -8,7 +8,7 @@
   pkg-config,
   wrapGAppsHook3,
   gtk3,
-  gtksourceview,
+  gtksourceview3,
   gtkspell3,
   webkitgtk_4_1,
   pandoc,
@@ -41,7 +41,7 @@ stdenv.mkDerivation (finalAttrs: {
 
   buildInputs = [
     gtk3
-    gtksourceview
+    gtksourceview3
     gtkspell3
     webkitgtk_4_1
     pandoc
diff --git a/pkgs/by-name/re/rednotebook/package.nix b/pkgs/by-name/re/rednotebook/package.nix
index 98a14bbca26d..00d1656a6082 100644
--- a/pkgs/by-name/re/rednotebook/package.nix
+++ b/pkgs/by-name/re/rednotebook/package.nix
@@ -6,7 +6,7 @@
   glib,
   gobject-introspection,
   gtk3,
-  gtksourceview,
+  gtksourceview3,
   pango,
   webkitgtk_4_1,
 }:
@@ -34,7 +34,7 @@ python3Packages.buildPythonApplication (finalAttrs: {
     gdk-pixbuf
     glib
     gtk3
-    gtksourceview
+    gtksourceview3
     pango
     webkitgtk_4_1
   ]
diff --git a/pkgs/by-name/ya/yad/package.nix b/pkgs/by-name/ya/yad/package.nix
index 52a921e783a0..a33b4978139a 100644
--- a/pkgs/by-name/ya/yad/package.nix
+++ b/pkgs/by-name/ya/yad/package.nix
@@ -8,7 +8,7 @@
   gettext,
   gspell,
   gtk3,
-  gtksourceview,
+  gtksourceview3,
   libappindicator,
   netpbm,
   webkitgtk_4_1,
@@ -40,7 +40,7 @@ stdenv.mkDerivation (finalAttrs: {
   buildInputs = [
     gspell
     gtk3
-    gtksourceview
+    gtksourceview3
     libappindicator
     webkitgtk_4_1
   ];
diff --git a/pkgs/development/ocaml-modules/lablgtk3/sourceview3.nix b/pkgs/development/ocaml-modules/lablgtk3/sourceview3.nix
index 0fa1039c62cb..9deb1b3a08ac 100644
--- a/pkgs/development/ocaml-modules/lablgtk3/sourceview3.nix
+++ b/pkgs/development/ocaml-modules/lablgtk3/sourceview3.nix
@@ -1,12 +1,12 @@
 {
   buildDunePackage,
-  gtksourceview,
+  gtksourceview3,
   lablgtk3,
 }:
 
 buildDunePackage {
   pname = "lablgtk3-sourceview3";
-  buildInputs = lablgtk3.buildInputs ++ [ gtksourceview ];
+  buildInputs = lablgtk3.buildInputs ++ [ gtksourceview3 ];
   propagatedBuildInputs = [ lablgtk3 ];
   inherit (lablgtk3)
     src
diff --git a/pkgs/top-level/aliases.nix b/pkgs/top-level/aliases.nix
index f3b83d44a238..c901e4d99f2a 100644
--- a/pkgs/top-level/aliases.nix
+++ b/pkgs/top-level/aliases.nix
@@ -1109,6 +1109,7 @@ mapAliases {
   gtklp = throw "'gtklp' has been removed, as it depended on GTK 2. Consider using 'system-config-printer' instead."; # Added 2026-05-22
   gtkmm2 = throw "'gtkmm2' has been removed as it depended on the deprecated gtk2. Consider using 'gtkmm3' or 'gtkmm4' instead."; # Added 2026-08-09
   gtkradiant = throw "'gtkradiant' has been removed, as it relies on gtk2"; # Added 2026-06-18
+  gtksourceview = throw "'gtksourceview' attribute has been removed from nixpkgs. Use a 'gtksourceview*' attribute with an explicit ABI version instead."; # Added 2026-09-04
   gtkspell2 = throw "'gtkspell2' has been removed as it depended on the deprecated GTK2 engine. Consider using 'gtkspell3' instead"; # Added 2026-07-23
   gtuber = throw "'gtuber' has been removed due to being discontinued by upstream."; # Added 2025-12-12
   gui-for-clash = throw "'gui-for-clash' has been removed, as it is unmaintained"; # Added 2026-05-28
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index c4e905968199..7f7c08e3e675 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -5795,8 +5795,6 @@ with pkgs;
 
   gtk-mac-integration-gtk3 = gtk-mac-integration;
 
-  gtksourceview = gtksourceview3;
-
   gtksourceview3 = callPackage ../development/libraries/gtksourceview/3.x.nix { };
 
   gtksourceview4 = callPackage ../development/libraries/gtksourceview/4.x.nix { };
@@ -9052,7 +9050,7 @@ with pkgs;
   };
 
   quodlibet-full = quodlibet.override {
-    inherit gtksourceview;
+    inherit gtksourceview3;
     kakasi = kakasi;
     keybinder3 = keybinder3;
     libappindicator = libappindicator;